Journal
TALANTA
Volume 247, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2022.123541
Keywords
Bioanalytical sensory system; Bacterial cells; Planktonic bacteria; Biofilm bacteria
Categories
Funding
- Ministry of Science and Higher Education of the Russian Federation
- Russian Science Foundation [22-24-00417]
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This article describes a new microfluidic bioanalytical sensory system for indicating biofilm formation. By recording cell-polarizability changes, this method can complete the analysis in 20-30 minutes without the need to grow bacterial biofilms in the sensor.
The formation of biofilms is a key factor that researchers must consider when they work with bacterial cultures. We describe a new microfluidic bioanalytical sensory system for indicating biofilm formation. The method is demonstrated with Pseudomonas bacteria as an example and is based on the real-time recording of cell-polarizability changes caused by an alternating electric field. Control experiments using phase-contrast microscopy and traditional microbiological plating were done that proved biofilms had formed. The physical picture was described of the sensor-signal changes during cell transition from planktonic to biofilm growth. This transition was indicated by the appearance of a peak-shaped signal at 500 kHz and by an increase in the recorded relaxation time. Phenomena of increase in the signal relaxation time from 2.4 s for planktonic to 25.4 s for biofilm cells. The proposed microfluidic sensor system for indicating biofilm formation holds much promise, because it ensures an analysis time of about 20-30 min. An added bonus is that for this system there is no need to grow bacterial biofilms in a sensor and the flow cell is reusable.
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