Journal
TALANTA
Volume 253, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2022.124047
Keywords
Photoacoustic biosensors; Rolling circle amplification; Single-nucleotide discrimination; SARS-CoV-2; N501Y
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In this study, a homogeneous and isothermal photoacoustic biosensor is demonstrated for rapid amplification and detection of synthetic DNA related to SARS-CoV-2. The biosensor achieves a sub-femtomolar detection limit within a total assay time of 80 min and is highly specific for single-nucleotide polymorphism discrimination. It is also robust against background DNA interference.
Rapid and accurate diagnosis of SARS-CoV-2 single-nucleotide variations is an urgent need for the initial detection of local circulation and monitoring the alternation of dominant variant. In this proof-of-concept study, a homogeneous and isothermal photoacoustic biosensor is demonstrated for rapid molecular amplification and detection of a synthetic DNA corresponding to SARS-CoV-2 spike N501Y. Branched rolling circle amplification produces single-stranded amplicons that can aggregate detection probe-modified AuNPs, which induces a strong photoacoustic signal at 640 nm due to both the surface plasmon resonance shift and the size-dependent effect of laser-induced nanobubbles, achieving a sub-femtomolar detection limit within a total assay time of 80 min. The limit of detection can be kept when measuring 5% serum samples. Moreover, the proposed biosensor is highly specific for single-nucleotide polymorphism discrimination and robust against background DNA.
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