Generation of two induced pluripotent stem cell lines (RCMGi005-A/B) from human skin fibroblasts of a cystic fibrosis patient with homozygous F508del mutation in CFTR gene

Induced pluripotent stem cells (iPSCs) was successfully generated from skin fibroblast obtained from patient with cystic fibrosis by using non-integrating, viral CytoTuneT (TM)-iPS 2.0 Sendai Reprogramming Kit, which contain three vectors preparation: polycistronic Klf4-Oct3/4-Sox2, cMyc, and Klf4. Created iPSC lines showed a normal karyotype, expressed pluripotency markers and demonstrated the potential to differentiate into three germ layers in spontaneous differentiation assay.

Automated summary

Induced pluripotent stem cells (iPSCs) were successfully generated from skin fibroblasts obtained from cystic fibrosis patients using the non-integrating, viral CytoTuneT (TM)-iPS 2.0 Sendai Reprogramming Kit, which contains three vectors: polycistronic Klf4-Oct3/4-Sox2, cMyc, and Klf4. The created iPSC lines had a normal karyotype, expressed pluripotency markers, and demonstrated the potential to differentiate into three germ layers in spontaneous differentiation assays.