Journal
STEM CELL RESEARCH
Volume 64, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.scr.2022.102935
Keywords
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Funding
- Sanming Project of Medicine in Shenzhen [SZSM201911004]
- Shenzhen Science and Technology Plan Basic Research Project [JCYJ20180307150408596, JCYJ20190809172403604]
- Natural Science Foundation of Guangdong Province [2019A1515110703]
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In this study, we reported a CD16A knockout human embryonic stem cell line generated through CRISPR/Cas9 gene editing. The mutated cell line exhibited normal cellular characteristics and provided insights into the role of CD16A in human cell development.
CD16A is a receptor for the Fc portion of immunoglobulin G, and is involved in the antibody dependent cellular cytotoxicity (ADCC) of nature killer cells(Zhu et al., 2020) and antibody dependent enhancement (ADE) of virus infections(Wan et al., 2020). However, the role of CD16A in human embryonic stem cell modeled development has been merely documented. Hence, to illustrate the role of CD16A in the human cell development, we reported a CD16A knockout human embryonic stem(hESC) cell line via CRISPR/Cas9 mediated gene knockout. The CD16A mutated cell line displayed normal karyotype, pluripotent stem cell marker gene expression and differentiation potential.
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