Journal
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
Volume 279, Issue -, Pages -Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2022.121275
Keywords
Raman spectroscopy; Tetrodotoxin(TTX); Aptasensor; Quantitative detection; Food safety
Categories
Funding
- Fujian Provincial Department of Science and Technology guiding Project [2020Y0019]
- Industry-university Cooperation Project of Fujian Provincial Department of Science and Technology [2020N5006]
- Opening Project of Jiangxi Province Key Laboratory of Polymer Micro/Nano Manufac-turing and Devices [PMND202007]
- Innovative Research Team in Science and Technology in Fujian Province University
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A novel dual-molecule calibration aptasensor based on Surface-enhanced Raman scattering (SERS) was developed for highly sensitive and specific detection of Tetrodotoxin (TTX). The aptasensor exhibited a wide linear range, low detection limit, and practical significance for TTX analysis in real seafood samples.
Tetrodotoxin (TTX) is one of the most lethal neurotoxins, so the reliable quantitative analysis of TTX is crucial for food and environmental safety monitoring. Herein, a novel dual-molecule calibration aptasensor was developed for detection of TTX based on Surface-enhanced Raman scattering (SERS). The adaptive surface has high affinity recognition sites for the target of interest, which ensures the high specificity and stability of the aptasensor. In addition, the uniquely labeled signal molecules located in the Raman silent region (1800-2400 cm(-1)) can avoid the interference of other exogenous biological signal molecules. Meanwhile, in quantitative analysis, the SERS signal generated by the reporter is calibrate in real time using the second-order peak of silicon molecule (Si). The detection linear range of the aptasensor was 0.0319 ng/mL-319.27 ng/mL, with a limit of detection (LOD) of 0.024 ng/mL and the excellent uniformity (RSD = 8.8%) for TTX detection. As a promising and versatile detection candidate, the ultra-sensitive quantitative detection aptasensor of TTX had important practical significance, which can offer more favorable persuasion for TTX analysis in real seafood samples. (C) 2022 Published by Elsevier B.V.
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