Journal
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
Volume 281, Issue -, Pages -Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2022.121595
Keywords
DNA; TERS probes; Nanospectroscopy; Atomic force microscopy; Principal Component Analysis
Categories
Funding
- National Science Centre, Poland [UMO-2018/31/B/ST4/02292]
- Jagiellonian Univeristy, Poland [2021-N17/MNS/03.41]
- Equipment Intervention Fund (EIF) - a part of the PRA SciMat - Strategic Program Excellence Initiative at the Jagiellonian University
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This work focuses on optimizing the TERS technique for efficient DNA measurements by preparing atomically-flat gold substrates, depositing DNA without fixatives, and optimizing TERS probe preparation. Through comprehensive comparison of different probes, reliable and reproducible TER spectra of DNA are obtained, providing a foundation for further research on DNA and its interaction with other biomolecules.
Tip-enhanced Raman (TER) spectroscopy combines the nanometric spatial resolution of atomic force microscopy (AFM) and the chemical sensitivity of Raman spectroscopy. Thus, it provides a unique possibility to obtain spectroscopic information on individual, nanometre-size molecules. The enhancement of Raman scattering crosssection requires modification of the AFM tip apex with a plasmonic nanostructure. Despite numerous advances of TERS research, attaining good reproducibility and stable enhancement is still challenging mainly due to the lack of optimized probes and sample preparation procedures. Moreover, current nanospectroscopic standard samples - carbon nanotubes (CNTs) have relatively simple chemical structure, and therefore, they are far from real-life analytes, especially biological samples. In this work we focus on the optimization of TERS technique for efficient DNA measurements, including: a preparation of atomically-flat gold substrates, fixative free deposition of DNA and optimization of TERS probe preparation. Here we demonstrate a comprehensive comparison of the efficacy of several types of TERS probes. Applying the systematic approach, we obtained reliable and reproducible TER spectra of DNA. Thus, we provide preparation procedures of a new standard TERS sample, TERS substrates and TERS probes. Our research provides a solid foundation for further research on DNA and its interaction with other biomolecules upon biologically significant processes such as DNA damage and repair.
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