Journal
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
Volume 279, Issue -, Pages -Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2022.121415
Keywords
Single-wall carbon nanohorns (SWCNHs); Energy transfer; Fluorescence quenching; Dopamine assay
Categories
Funding
- Middle-aged Backbone Personnel Training Program of Shenyang Pharmaceutical University [ZQN2016011]
- Scientific Research Fund of Liaoning Provincial Edu-cation Department [2020LZD02]
- Project of Shenyang Key Labora-tory of Functional Drug Carrier Materials [19-110-4-08]
Ask authors/readers for more resources
A fast and simple aptamer-based fluorescence strategy using single-wall carbon nanohorns (SWCNHs) as a quencher for dopamine detection has been developed in this study. The innovative detection system shows a detection limit of 5 μM and a linear detection range of 0.02-2.20 mM, and has been successfully applied to detect dopamine in spiked serum samples.
Dopamine (DBA) as an important biomarker, plays a crucial role in disease diagnosis. In this study, we have developed a fast and simple aptamer-based fluorescence strategy which used single-wall carbon nanohorns (SWCNHs) as a quencher for dopamine detection. SWCNHs were negatively charged after pretreated, which improved its dispersion in solution. 5-carboxy-fluorescein (FAM) was used to label dopamine aptamer. In the absence of dopamine, FAM-modified aptamer could be absorbed onto the SWCNHs surface due to pi-pi interaction, resulting in the fluorescence intensity decreased. Dopamine could specifically bind with FAM-DNA to form G-quadruplex, which could not be absorbed onto the surface of SWCNHs. Hence, the fluorescence of FAM-DNA recovered, and the fluorescent intensity as a function of different concentrations of dopamine was measured. We obtained a detection limit of 5 mu M for this detection system with a linear detection range of 0.02-2.20 mM. Furthermore, the feasibility of the innovative detection system has been verified by detecting dopamine in spiked serum samples.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available