In vitro cytotoxic activity-guided isolation studies on the underground parts of Valeriana speluncaria Boiss

Two unreported neolignans, speluncarins A (1) and B (2) together with seven known compounds (3-8, 13) were isolated from the underground parts of Valeriana speluncaria Boiss through cytotoxic activity-guided fractionation method. The crude extract, subextracts, main fractions and isolates were evaluated for their in vitro cytotoxic activities against MCF-7 and U87 cancer cell lines as well as HUVEC cell line by MTS assay. Although the crude EtOH extract, CHCl3 and EtOAc subextracts as well as some fractions exhibited remark-able cytotoxic effect against tested cancer cell lines, the purified compounds did not demonstrate promising activity in the tested concentrations. Besides these studies, five known lignan glucosides (8-12) were obtained from the inactive n-BuOH subextract. The structures of the isolates were elucidated by extensive 1D and 2D NMR spectroscopy and HR-MS analysis. This is the first phytochemical and bioactivity study on V. speluncaria, an endemic species to Turkey. (C) 2022 SAAB. Published by Elsevier B.V. All rights reserved.

Automated summary

This study conducted phytochemical and bioactivity research on Valeriana speluncaria, an endemic species to Turkey, and isolated two unreported neolignans and seven known compounds through cytotoxic activity-guided fractionation method.