4.6 Article

Diurnal variation of clock genes expression and other sleep-wake rhythm biomarkers among acute ischemic stroke patients

Journal

SLEEP MEDICINE
Volume 99, Issue -, Pages 1-10

Publisher

ELSEVIER
DOI: 10.1016/j.sleep.2022.06.023

Keywords

Stroke; Clock genes; Melatonin; Cortisol; Circadian; Cerebrovascular; Actigraphy; Polysomnography

Funding

  1. Science Fund of Lithuanian University of Health Sciences

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This study is the first to investigate clock genes expression profiles in ischemic stroke patients, revealing specific circadian desynchrony features in different loops of the molecular circadian clock system.
There is accumulating evidence about sleep-wake rhythm disturbances as potential modifiable risk factors of both incident and recurrent stroke and less favorable outcomes after stroke. To our best knowledge this is the first study designed to investigate clock genes expression profiles in ischemic stroke patients and their relations to other biological and behavioral sleep-wake rhythm biomarkers, sleep structural and clinical stroke features. Altogether, 27 ischemic stroke patients (20 males) with the median age of 56 years and 25 gender and age matched controls were investigated with neurological and objective examination, scales, polysomnography, actigraphy and 24-h blood sampling for melatonin and clock genes profiles. Median melatonin plasma concentrations at four time points at 7, 11 p.m., 3 a.m. and 12 p.m. did not differ significantly between patients and controls, only early morning melatonin concentration at 7 a.m. was significantly lower and cortisol plasma concentration - significantly higher among stroke patients. All four clock genes (ARNTL (BMAL1), NR1D1 (Rev-erba/b), PER1, and PER3) showed significant time-of-day variation in both patients' and controls' groups, except expression of NR1D1 (Reverba/b) at 7 a.m. and PER1 at 12 p.m. differed significantly. In conclusion, acute ischemic stroke patients tended to preserve most of diurnal variation of sleep-wake rhythm molecular patterns. Nevertheless, early morning time point showing higher cortisol and lower melatonin concentrations and lower NR1D1 (Rev-erba/b) expression, as well as lower PER1 midday expression reflect specific circadian desynchrony features in different loops of the molecular circadian clock system. (c) 2022 Elsevier B.V. All rights reserved.

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