A strategy of fluorescence-visualization dual channel for highly sensitive and rapid detection of phosphate biomarkers based on stimulus-responsive infinite coordination polymers

The novel stimulus-responsive host-guest nanoparticles were prepared based on lanthanide infinite coordination polymer (COU@Eu-ICPs) for rapid and point-of-care analysis of phosphate (Pi) biomarkers. The obtained COU@Eu-ICPs, which have the host-guest structure, were applied to high-sensitive detection of Pi biomarkers by the means of ratiometric fluorescence-visualization dual channel. The established method has the advantages of low detection limit (51 nM), wide linear range (0.25-5 mu M) and rapid response to Pi within 3 min. The developed sensor was used to detect Pi in normal human urine and serum samples, with the recoveries of 96.04-106.3%. Furthermore, the fluorescent color changes of COU@Eu-ICPs from blue to red can be used to visually inspect Pi by the naked eyes. More importantly, a portable test strip based on COU@Eu-ICPs was fabricated and successfully applied to the rapid visual detection of Pi in human urine and serum samples.

Automated summary

Novel stimulus-responsive host-guest nanoparticles were prepared for rapid and point-of-care analysis of phosphate (Pi) biomarkers based on lanthanide infinite coordination polymer (COU@Eu-ICPs). The developed method showed low detection limit, wide linear range, and rapid response to Pi, and the presence of Pi could be visually inspected.