Genome- and transcriptome-wide identification and analysis of B3 superfamily members and their association with salt stress response in the common bean (Phaseolus vulgaris L)

The plant-specific transcription factor B3 superfamily members mediate plant growth, development, and stress response. Although the B3 gene has been studied in various plants, its role in the common bean (Phaseolus vulgaris L) is unknown. In this study, 110 PvB3 genes were identified from the common bean genome and analyzed based on the phylogeny, gene structure, motifs, cis-regulatory elements, collinearity, and Ka/Ks. PvB3s were divided into four subgroups based on motifs and gene structure. The cis-element composition of the most PvB3 were involved in hormone production, abiotic stress response, and germination. Most PvB3 were highly expressed in the vigorously growth tissue of common bean, such as nodules, young pods, and young trifoliate. Moreover, RNA-seq results showed that PvB3-001/010/027/032/047/048/059/090 were enriched in trypto-phan metabolism pathway (involves in AUX/IAA, GH3, and SAUR) of common bean under salt stress. Notably, exogenous IAA treatment could increase endogenous IAA content and the number of lateral roots, while altering the expression level of PvB3s in salt-sensitive common bean variety under salt stress. Taken together, the result indicated that PvB3 transcription factors may be involved in common bean salt stress response by participating in the synthesis and metabolic regulation of IAA. This study provides a theoretical basis for further study on the role of PvB3s in common bean abiotic stresses response.

Automated summary

The PvB3 transcription factors may be involved in the salt stress response of common bean by participating in the synthesis and metabolic regulation of IAA.