4.5 Article

Host protein kinases required for SARS-CoV-2 nucleocapsid phosphorylation and viral replication

Related references

Note: Only part of the references are listed.
Article Biochemistry & Molecular Biology

The PRIDE database resources in 2022: a hub for mass spectrometry-based proteomics evidences

Yasset Perez-Riverol et al.

Summary: PRIDE is the largest data repository of mass spectrometry-based proteomics data in the world, with around 500 datasets submitted per month. In addition to continuous improvements in data pipelines and infrastructure, PRIDE has developed the Spectra Archive and MAGE-TAB file format to enhance sample metadata annotation and access to mass spectra.

NUCLEIC ACIDS RESEARCH (2022)

Article Biochemistry & Molecular Biology

Identification of Required Host Factors for SARS-CoV-2 Infection in Human Cells

Zharko Daniloski et al.

Summary: Researchers conducted a genome-scale CRISPR screen to identify host factors crucial for SARS-CoV-2 infection, highlighting genes related to ATPase proton pump, Retromer, and Commander complexes. Validating these gene targets through multiple methods, they found shared transcriptional changes in cholesterol biosynthesis when top-ranked genes were lost. Loss of RAB7A was shown to reduce viral entry by sequestering the ACE2 receptor inside cells, underscoring its importance early in the viral infection process.
Article Multidisciplinary Sciences

Targeting the coronavirus nucleocapsid protein through GSK-3 inhibition

Xiaolei Liu et al.

Summary: The nucleocapsid protein (N) of coronaviruses is essential for viral replication, and the phosphorylation of N by glycogen synthase kinase 3 (GSK-3) is crucial for its function. The study found that the N protein of SARS-CoV-2 may be sensitive to GSK-3 inhibitors like lithium, leading to reduced risk of COVID-19 in patients taking lithium. Inhibition of GSK-3 impairs viral transcription and replication, suggesting that targeting GSK-3 could be a potential approach to treat COVID-19 and future coronavirus outbreaks.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2021)

Article Cell Biology

SARS-CoV-2 Nucleocapsid Protein Targets RIG-I-Like Receptor Pathways to Inhibit the Induction of Interferon Response

Soo Jin Oh et al.

Summary: The study demonstrates that the nucleocapsid protein (N) of SARS-CoV-2 can suppress the host antiviral immune response, inhibiting interferon production and signaling.

CELLS (2021)

Article Multidisciplinary Sciences

Multilevel proteomics reveals host perturbations by SARS-CoV-2 and SARS-CoV

Alexey Stukalov et al.

Summary: The study reports a concurrent multi-omics analysis of SARS-CoV-2 and SARS-CoV, revealing both distinct and common molecular mechanisms between these closely related coronaviruses, as well as their impact on the host cell proteome. The results of this study may provide insights into the design of virus- and host-directed therapies for these coronaviruses.

NATURE (2021)

Article Cell Biology

Targeting liquid-liquid phase separation of SARS-CoV-2 nucleocapsid protein promotes innate antiviral immunity by elevating MAVS activity

Shuai Wang et al.

Summary: Wang et al. found that the nucleocapsid protein of SARS-CoV-2 forms phase-separated condensates to repress antiviral immunity by inhibiting ubiquitination and aggregation of mitochondrial antiviral-signalling protein. They also identified a key acetylation site and mutations that impair the protein's phase separation with RNA, and developed a peptide disrupting this process to inhibit viral replication and enhance antiviral immunity.

NATURE CELL BIOLOGY (2021)

Article Multidisciplinary Sciences

Characterization of SARS-CoV-2 nucleocapsid protein reveals multiple functional consequences of the C-terminal domain

Chao Wu et al.

Summary: The study characterizes the biochemical properties of the nucleocapsid protein N encoded by SARS-CoV-2 and highlights the value of using N domains as highly specific and sensitive diagnostic markers. The research provides insights into N oligomerization, RNA binding, phosphorylation regulation, and immunogenicity of different N domains.

ISCIENCE (2021)

Article Biochemistry & Molecular Biology

RNA-induced liquid phase separation of SARS-CoV-2 nucleocapsid protein facilitates NF-κB hyper-activation and inflammation

Yaoxing Wu et al.

Summary: The study reveals that the nucleocapsid (N) protein of SARS-CoV-2 promotes the activation of the NF-κB signaling pathway, leading to inflammatory responses. Inhibiting liquid-liquid phase separation can weaken the regulatory function of this protein, offering a potential therapeutic target for severe pneumonia caused by COVID-19.

SIGNAL TRANSDUCTION AND TARGETED THERAPY (2021)

Article Medicine, General & Internal

Early Transmission Dynamics in Wuhan, China, of Novel Coronavirus-Infected Pneumonia

Qun Li et al.

NEW ENGLAND JOURNAL OF MEDICINE (2020)

Article Biochemistry & Molecular Biology

Imbalanced Host Response to SARS-CoV-2 Drives Development of COVID-19

Daniel Blanco-Melo et al.

Article Multidisciplinary Sciences

A SARS-CoV-2 protein interaction map reveals targets for drug repurposing

David E. Gordon et al.

NATURE (2020)

Review Immunology

COVID-19 pathophysiology: A review

Koichi Yuki et al.

CLINICAL IMMUNOLOGY (2020)

Letter Oncology

COVID-19 in lung cancer patients receiving ALK/ROS1 inhibitors

Alessandro Leonetti et al.

EUROPEAN JOURNAL OF CANCER (2020)

Article Biochemistry & Molecular Biology

The Global Phosphorylation Landscape of SARS-CoV-2 Infection

Mehdi Bouhaddou et al.

Article Biochemistry & Molecular Biology

Growth Factor Receptor Signaling Inhibition Prevents SARS-CoV-2 Replication

Kevin Klann et al.

MOLECULAR CELL (2020)

Article Biochemistry & Molecular Biology

SR/RS Motifs as Critical Determinants of Coronavirus Life Cycle

Eleni Nikolakaki et al.

FRONTIERS IN MOLECULAR BIOSCIENCES (2020)

Article Biochemistry & Molecular Biology

Phosphoregulation of Phase Separation by the SARS-CoV-2 N Protein Suggests a Biophysical Basis for its Dual Functions

Christopher R. Carlson et al.

MOLECULAR CELL (2020)

Article Biochemistry & Molecular Biology

Actionable Cytopathogenic Host Responses of Human Alveolar Type 2 Cells to SARS-CoV-2

Ryan M. Hekman et al.

MOLECULAR CELL (2020)

Article Multidisciplinary Sciences

Nucleocapsid protein of SARS-CoV-2 phase separates into RNA-rich polymerase-containing condensates

Adriana Savastano et al.

NATURE COMMUNICATIONS (2020)

Article Immunology

Severe Acute Respiratory Syndrome Coronavirus 2 from Patient with Coronavirus Disease, United States

Jennifer Harcourt et al.

EMERGING INFECTIOUS DISEASES (2020)

Article Biochemistry & Molecular Biology

Distinct mechanisms govern the phosphorylation of different SR protein splicing factors

Yunxin Long et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2019)

Article Biochemistry & Molecular Biology

SRPKIN-1: A Covalent SRPK1/2 Inhibitor that Potently Converts VEGF from Pro-angiogenic to Anti-angiogenic Isoform

John M. Hatcher et al.

CELL CHEMICAL BIOLOGY (2018)

Article Biochemistry & Molecular Biology

Post-transcriptional Regulation of De Novo Lipogenesis by mTORC1-S6K1-SRPK2 Signaling

Gina Lee et al.

Article Biochemical Research Methods

ggseqlogo: a versatile R package for drawing sequence logos

Omar Wagih

BIOINFORMATICS (2017)

Article Biochemistry & Molecular Biology

Development of Potent, Selective SRPK1 Inhibitors as Potential Topical Therapeutics for Neovascular Eye Disease

Jennifer Batson et al.

ACS CHEMICAL BIOLOGY (2017)

Article Biochemical Research Methods

A Triple Knockout (TKO) Proteomics Standard for Diagnosing Ion Interference in Isobaric Labeling Experiments

Joao A. Paulo et al.

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY (2016)

Article Biochemistry & Molecular Biology

limma powers differential expression analyses for RNA-sequencing and microarray studies

Matthew E. Ritchie et al.

NUCLEIC ACIDS RESEARCH (2015)

Review Pharmacology & Pharmacy

Glycogen synthase kinase-3 (GSK3): Regulation, actions, and diseases

Eleonore Beurel et al.

PHARMACOLOGY & THERAPEUTICS (2015)

Review Virology

The Coronavirus Nucleocapsid Is a Multifunctional Protein

Ruth McBride et al.

VIRUSES-BASEL (2014)

Article Biochemistry & Molecular Biology

Splicing Kinase SRPK1 Conforms to the Landscape of Its SR Protein Substrate

Brandon E. Aubol et al.

BIOCHEMISTRY (2013)

Article Biochemical Research Methods

Comet: An open-source MS/MS sequence database search tool

Jimmy K. Eng et al.

PROTEOMICS (2013)

Article Chemistry, Analytical

Increasing the Multiplexing Capacity of TMTs Using Reporter Ion Isotopologues with Isobaric Masses

Graeme C. McAlister et al.

ANALYTICAL CHEMISTRY (2012)

Article Biochemical Research Methods

MS3 eliminates ratio distortion in isobaric multiplexed quantitative proteomics

Lily Ting et al.

NATURE METHODS (2011)

Article Microbiology

Inhibition of Hepatitis C Virus Replication by a Specific Inhibitor of Serine-Arginine-Rich Protein Kinase

Yuko Karakama et al.

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY (2010)

Article Biochemistry & Molecular Biology

A Tissue-Specific Atlas of Mouse Protein Phosphorylation and Expression

Edward L. Huttlin et al.

Article Biotechnology & Applied Microbiology

Regulation of the subcellular distribution of key cellular RNA-processing factors during permissive human cytomegalovirus infection

Charla E. Gaddy et al.

JOURNAL OF GENERAL VIROLOGY (2010)

Article Biochemistry & Molecular Biology

The SR-rich motif in SARS-CoV nucleocapsid protein is important for virus replication

Shaun Tylor et al.

CANADIAN JOURNAL OF MICROBIOLOGY (2009)

Article Biochemistry & Molecular Biology

Coronavirus N Protein N-Terminal Domain (NTD) Specifically Binds the Transcriptional Regulatory Sequence (TRS) and Melts TRS-cTRS RNA Duplexes

Nicholas E. Grossoehme et al.

JOURNAL OF MOLECULAR BIOLOGY (2009)

Article Biochemistry & Molecular Biology

Structurally unique yeast and mammalian serine-arginine protein kinases catalyze evolutionarily conserved phosphorylation reactions

Randall Lukasiewicz et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2007)

Article Biotechnology & Applied Microbiology

A probability-based approach for high-throughput protein phosphorylation analysis and site localization

Sean A. Beausoleil et al.

NATURE BIOTECHNOLOGY (2006)

Article Multidisciplinary Sciences

Utilization of host SR protein kinases and RNA-splicing machinery during viral replication

Takeshi Fukuhara et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2006)

Article Cell Biology

Regulated cellular partitioning of SR protein-specific kinases in mammalian cells

JH Ding et al.

MOLECULAR BIOLOGY OF THE CELL (2006)

Article Biochemistry & Molecular Biology

Mass spectrometric and kinetic analysis of ASF/SF2 phosphorylation by SRPK1 and Clk/Sty

A Velazquez-Dones et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2005)

Article Biochemical Research Methods

A rapid method for determining protein kinase phosphorylation specificity

JE Hutti et al.

NATURE METHODS (2004)

Article Biochemistry & Molecular Biology

Structure of the N-terminal RNA-binding domain of the SARS CoV nucleocapsid protein

QL Huang et al.

BIOCHEMISTRY (2004)

Article Biotechnology & Applied Microbiology

Determination of protease cleavage site motifs using mixture-based oriented peptide libraries

BE Turk et al.

NATURE BIOTECHNOLOGY (2001)