4.6 Article

Metabolic profiling of preovulatory follicular fluid in jennies

Journal

RESEARCH IN VETERINARY SCIENCE
Volume 153, Issue -, Pages 127-136

Publisher

ELSEVIER SCI LTD
DOI: 10.1016/j.rvsc.2022.10.026

Keywords

Donkey; Equids; Ovary; Preovulatory follicle; Follicular fluid; Metabolomic; Metabolites

Funding

  1. Italian Ministry for Education, University and Research [CUP_C46C18000530001]
  2. Spanish Ministry of Science, Innovation and Universities [BES-2017-081962]
  3. Secretary of Higher Education, Science, Technology and Innovation (SENESCYT) , Ecuador [CZ02-000507-2019]
  4. Ministry of Science and Innovation, Spain [AGL2017-88329-R]
  5. Catalan Agency for Management of University
  6. Catalan Agency for Management of University and Research Grants, Regional Government of Catalonia, Spain [2017-SGR-1229]
  7. Catalan Institution for Research and Advanced Studies, Spain (ICREA)

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The aim of this study was to determine the metabolic profile of preovulatory follicular fluid (PFF) in jennies. The results revealed a unique metabolic composition of jennies' PFF, different from that of other closely related species. These findings contribute to the understanding of oocyte maturation in jennies and provide a basis for establishing in vitro reproductive techniques in this species.
Follicular fluid is formed from the transudation of theca and granulosa cells in the growing follicular antrum. Its main function is to provide an optimal intrafollicular microenvironment to modulate oocyte maturation. The aim of this study was to determine the metabolomic profile of preovulatory follicular fluid (PFF) in jennies. For this purpose, PFF was collected from 10 follicles of five jennies in heat. Then, PFF samples were analysed by nuclear magnetic resonance (NMR) and heteronuclear single quantum correlation (2D 1H/13C HSQC). Our study revealed the presence of at least 27 metabolites in the PFF of jennies (including common amino acids, carboxylic acids, amino acid derivatives, alcohols, saccharides, fatty acids, and lactams): 3-hydroxybutyrate, acetate, alanine, betaine, citrate, creatine, creatine phosphate, creatinine, ethanol, formate, glucose, glutamine, glycerol, glycine, hippurate, isoleucine, lactate, leucine, lysine, methanol, phenylalanine, proline, pyruvate, threonine, tyrosine, valine, and tau-methylhistidine. The metabolites found here have an important role in the oocyte development and maturation, since the PFF surrounds the follicle and provides it with the needed nutrients. Our results indicate a unique metabolic profile of the jennies PFF, as it differs from those previously observed in the PFF of the mare, a phylogenetically close species that is taken as a reference for establishing reproductive biotechnology techniques in donkeys. The metabolites found here also differ from those described in the TCM-199 medium enriched with fetal bovine serum (FBS), which is the most used medium for in vitro oocyte maturation in equids. These dif-ferences would suggest that the established conditions for in vitro maturation used so far may not be suitable for donkeys. By providing the metabolic composition of jenny PFF, this study could help understand the physiology of oocyte maturation as a first step to establish in vitro reproductive techniques in this species.

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