Larvicidal and pupicidal activity of crude ethyl acetate extract fraction-7a of Cymodocea serrulata on Culex quinquefasciatus

Finding a potential bioactive compound against a specific mosquito vector is an urgent requirement. Thus, this research was designed to separate the significant compounds present in the crude ethyl acetate extract of Cymodocea serrulata through Thin Layer Chromatography (TLC), Column Chromatography (CC), and High Per-formance Liquid Chromatography (HPLC). Furthermore, the larvicidal and pupicidal activity of different concentrations (100, 300, 500, 700, and 900 g mL(-1)) of separated fraction against 4th instar larvae of Culex quinquefasciatus in 24 h of treatment. Interestingly, 7 fractions were obtained from crude ethyl acetate extract by TLC. One fraction (7a) from out of 7 was further separated into fractions 7a and 7b through CC and preparative TLC. Furthermore, the HPLC analysis result revealed that the fraction-7a contained 2 major peaks and 25 minor peaks. The fraction 7a was selected based on the R-f value and examined their larvicidal and pupicidal activity on 4th instar larvae and pupa of C. quinquefasciatus. Interestingly, the increased concentration (900 mu g mL(-1)) of fraction-7a of ethyl acetate extracts of C. serrulata showed excellent larvicidal and pupicidal activities as 98.66 +/- 1.88 % and 98 +/- 5.65 %, respectively. These findings imply that the fraction 7a of C. serrulata ethyl acetate extract could be used as a mosquito vector control agent.

Automated summary

This study aimed to isolate significant compounds from the crude ethyl acetate extract of Cymodocea serrulata and evaluate their larvicidal and pupicidal activity against Culex quinquefasciatus. The results showed that fraction 7a had strong larvicidal and pupicidal activities and could be used as a mosquito vector control agent.