Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 119, Issue 41, Pages -Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2205874119
Keywords
glycine transporters; ion-coupled transporter; electrophysiology; thermodynamic; NMDAR
Categories
Funding
- National Institute of Health and Medical Research (INSERM)
- National Centre for Scientific Research (CNRS)
- La Fondation pour la Recherche Medicale Equipe FRM grant [DEQ20140329498]
- Agence Nationale de la Recherche [ANR-10-LABX-54 MEMOLIFE, ANR-11-IDEX-0001-02]
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ATB(0,+) is a member of the SLC6 family that functions as an amino acid transporter with broad substrate specificity. It exhibits a 3:1:1 Na+ :Cl- :Gly stoichiometry and behaves as a unidirectional transporter, with virtually no efflux of glycine at negative potentials. Its charge movement analysis reveals a higher affinity for sodium compared to GlyT2, and a gating mechanism that locks sodium into the transporter at depolarized potentials.
ATB(0,+) (SLC6A14) is a member of the amino acid transporter branch of the SLC6 family along with GlyT1 (SLC6A9) and GlyT2 (SLC6A5), two glycine-specific transporters coupled to 2:1 and 3:1 Na+ :Cl-, respectively. In contrast, ATB(0,+) exhibits broad substrate specificity for all neutral and cationic amino acids, and its ionic coupling remains unsettled. Using the reversal potential slope method, we demonstrate a 3:1:1 Na+ :Cl- :Gly stoichiometry for ATB(0,+) that is consistent with its 2.1 e/Gly charge coupling. Like GlyT2, ATB(0,+) behaves as a unidirectional transporter with virtually no glycine efflux at negative potentials after uptake, except by heteroexchange as remarkably shown by leucine activation of NMDARs in Xenopus oocytes coexpressing both membrane proteins. Analysis and computational modeling of the charge movement of ATB(0,+) reveal a higher affinity for sodium in the absence of substrate than GlyT2 and a gating mechanism that locks Na+ into the apo-transporter at depolarized potentials. A 3:1 Na+ :Cl- stoichiometry justifies the concentrative transport properties of ATB0,+ and explains its trophic role in tumor growth, while rationalizing its phylogenetic proximity to GlyT2 despite their extreme divergence in specificity.
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