Journal
PLANT PHYSIOLOGY AND BIOCHEMISTRY
Volume 186, Issue -, Pages 310-321Publisher
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.plaphy.2022.07.020
Keywords
Brassica napus L; CAPS marker; Genome-wide analysis; Serine carboxypeptidases-like protein
Categories
Funding
- National Program on Basic Research of China [2015CB150201]
- Natural Science Foundation of Chongqing, China [cstc2019jcyj-bshX0055, cstc2019jcyj-zdxmX0012]
Ask authors/readers for more resources
In this study, a total of 117 putative SCPL genes were identified in Brassica napus, and their distribution, evolution and functions were investigated. The results showed that the SCPL gene family was amplified during polyploidization, and the genes in the same group displayed high conservation. Furthermore, the study suggested the important role of the BnSCPL52 gene in seed yield and silique length in rapeseed.
The serine carboxypeptidase-like protein (SCPL) family plays a key part in plant growth, development and stress responses. However, the serine carboxypeptidase-like (SCPL) proteins in Brassica napus L. (B. napus) have not been reported yet. Here, we identified a total of 117 putative SCPL genes in B. napus, which were unevenly distributed on all 19 chromosomes and were divided into three groups (carboxypeptidase I to III) according to their phylogenetic relationships. Synteny and duplication analysis revealed that the SCPL gene family of B. napus was amplified during allopolyploidization, in which the whole genome triplication and dispersed duplication played critical roles. After the separation of Brassica and Arabidopsis lineages, orthologous gene analysis showed that many SCPL genes were lost during the evolutionary process in B. rapa, B. oleracea and B. napus. Subse-quently, the analyses of the gene structure, conserved motifs, cis-element and expression patterns showed that the members in the same group were highly conserved. Furthermore, candidate gene based association study suggested the role of BnSCPL52 in controlling seed number per silique, seed weight and silique length and a CAPS marker was developed to distinguish different haplotypes. Our results provide an overview of rapeseed SCPL genes that enable us for further functional research and benefit the marker-assisted breeding in Brassica napus.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available