Journal
PLANT PHYSIOLOGY AND BIOCHEMISTRY
Volume 186, Issue -, Pages 220-231Publisher
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.plaphy.2022.07.023
Keywords
TPS; Sesame; Fatty acid; Oil content; GWAS; Candidate gene; Transcriptome
Categories
Funding
- Agricultural Science and Technology Innovation Project of the Chinese Academy of Agricultural Sciences [CAAS-ASTIP-2016-OCRI]
- Key Research Projects of Hubei province [2020BBA045, 2020BHB028]
- Science and Technology Innovation Project of Hubei province [2021-620-000-001-035]
- Fundamental Research Funds for Central Non-profit Scientific Institution [Y2022XK11]
- Open Project of Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, P. R. China [KF2020004]
- China Agriculture Research System [CARS -14]
- Shanghai Science and Technology Committee Rising -Star Program [19QA1406500]
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The regulatory mechanisms of fatty acid biosynthesis and triacylglycerols assembly were investigated in sesame using gas chromatography and whole-genome resequencing. A significant gene, SiTPS1, was identified as a key regulator of FAs and TAGs metabolism in sesame. This study provides valuable resources for improving oil content and quality in sesame and other crops.
The regulatory mechanisms of fatty acid (FA) biosynthesis and triacylglycerols (TAGs) assembly remain largely misunderstood in sesame. Gas chromatography was used to analyze the natural variation in FA compositions and oil content (OC) in 400 sesame accessions grown in three different environments. The phenotypic data was associated with the newly released SNP data from whole-genome resequencing, and 43 significant loci for FA and OC were identified. Comparative transcriptomics analysis of high-OC and low-OC materials was performed, and 515 differentially expressed genes (DEGs) were identified across three seed developmental stages. By integrating the genome-wide association study (GWAS) and DEGs analysis, twenty candidate genes were identified, of which SiTPS1 (trehalose-6-phosphate synthase 1) has emerged as a key regulatory gene of FAs and TAGs metabolism in sesame. Overexpression of SiTPS1 in transgenic Arabidopsis influenced FA composition and significantly increased OC. Our study provides resources for the markers-based improvement of OC and quality in sesame and other crops.
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