Journal
PLANT PHYSIOLOGY
Volume 190, Issue 3, Pages 1792-1805Publisher
OXFORD UNIV PRESS INC
DOI: 10.1093/plphys/kiac384
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Funding
- United States Department of Agriculture-Agricultural Research Service CRIS project [605421000-018-00D]
- Cotton Incorporated [58-6435-2-663]
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In this study, it was found that the elongation of fiber cells in the Ligon lintless-2 (Li-2) cotton mutant is inhibited by siRNAs. This inhibition is caused by a large structural rearrangement at the end of chromosome D13, which leads to the production of self-complementary transcripts and generation of siRNAs. This finding reveals the regulatory mechanism of the Ran Binding Protein 1 family in cotton fiber growth.
Most cultivated cotton (Gossypium hirsutum L.) varieties have two types of seed fibers: short fuzz fiber strongly adhered to the seed coat, and long lint fiber used in the textile industry. The Ligon lintless-2 (Li-2) cotton mutant has a normal vegetative phenotype but produces very short lint fiber on the seeds. The Li-2 mutation is controlled by a single dominant gene. We discovered a large structural rearrangement at the end of chromosome D13 in the Li-2 mutant based on whole-genome sequencing and genetic mapping of segregating populations. The rearrangement contains a 177-kb deletion and a 221-kb duplication positioned as a tandem inverted repeat. The gene Gh_D13G2437 is located at the junction of the inverted repeat in the duplicated region. During transcription such structure spontaneously forms self-complementary hairpin RNA of Gh_D13G2437 followed by production of small interfering RNA (siRNA). Gh_D13G2437 encodes a Ran-Binding Protein 1 (RanBP1) that preferentially expresses during cotton fiber elongation. The abundance of siRNA produced from Gh_D13G2437 reciprocally corresponds with the abundance of highly homologous (68%-98% amino acid sequence identity) RanBP1 family transcripts during fiber elongation, resulting in a shorter fiber phenotype in the Li-2. Overexpression of Gh_D13G2437 in the Li-2 mutant recovered the long lint fiber phenotype. Taken together, our findings revealed that siRNA-induced silencing of a family of RanBP1s inhibit elongation of cotton fiber cells in the Li-2 mutant. A deletion/duplication in the Ligon lintless-2 locus induces siRNAs from self-complementary transcripts of Ran Binding Protein 1 that inhibit cotton fiber cell elongation.
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