Extensive cross-talk among stress-regulated protective metabolites, biogenic-amines and phytohormone-signalling, co-ordinated by dopamine-mediated seed-priming, governs tolerance against fluoride stress in rice

Key message Seed priming with dopamine reduced fluoride bioaccumulation, induced endogenous dopamine level, thereby orchestrating phytohormone homeostasis and biogenic amine metabolism, and modulating osmolyte and antioxidant machinery to enhance fluoride tolerance in rice. The aim of this study was to decipher the efficacy of seed priming with dopamine in curtailing the adverse impacts of fluoride toxicity in rice seedlings. Fluoride-stressed seedlings exhibited severe growth retardation, high fluoride bioaccumulation, electrolyte leakage and marked cellular injuries. Dopamine priming stimulated the overall physiological growth parameters during stress, via reduced formation of H2O2, malondialdehyde and methylglyoxal, due to lesser fluoride-accumulation. Fluoride stress-induced endogenous dopamine level was further induced upon dopamine priming, marked by the up regulated DOPA decarboxylase expression. Additionally, dopamine treatment led to escalated activity of catalase, superoxide dismutase and glutathione peroxidase in the stressed seedlings, concomitant with altered CAT, SOD and GPX expression. The higher accumulation of protective osmolytes (proline and total amino acids) and non-enzymatic antioxidants (phenolics, flavonoids, anthocyanins, glutathione and carotenoids), upon dopamine priming, during fluoride stress, could be linked with the altered expression pattern of the respective genes. Dopamine promoted active utilization of the biogenic amine (polyamines and -amino butyric acid) pools for toxicity mitigation, correlated with the modulation of the concerned enzyme activity and gene expression. Dopamine stimulated the accumulation of phytohormones like gibberellin and salicylic acid, via inducing the biosynthetic genes like gibberellin-3-oxidase (GA3ox) and isochorismate synthase (ICS), respectively, while depreciating the abscisic acid and melatonin level during fluoride stress. To our knowledge, this is the first documented report for the remedial role of dopamine priming against fluoride stress in any plant species. This study will open new arenas in sustainable agriculture for the exploitation of this pulsating biomolecule against fluoride stress.

Automated summary

Seed priming with dopamine reduces fluoride bioaccumulation and enhances fluoride tolerance in rice by stimulating growth, modulating phytohormone homeostasis, and promoting antioxidant and osmolyte metabolism. Dopamine also promotes the utilization of biogenic amines for toxicity mitigation and alters the expression of related genes. This study provides the first documented evidence of the remedial role of dopamine priming against fluoride stress in plants, opening up new possibilities for sustainable agriculture.