Journal
PARASITOLOGY RESEARCH
Volume 121, Issue 12, Pages 3663-3670Publisher
SPRINGER
DOI: 10.1007/s00436-022-07693-3
Keywords
Real time PCR; Trichomonas gypaetinii; Trichomonas gallinae; Sensitivity; Oropharyngeal trichomonosis; Avian trichomonosis
Categories
Funding
- CRUE-CSIC agreement
- Springer Nature
- Prevalencia de tricomonosis en el aguila azor perdicera [415-2018]
- LIFE [NAT/ES/000701]
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This study evaluated an adapted real-time PCR method for diagnosing different genotypes and species of avian oropharyngeal trichomonads and found multiple genotypes and species in Bonelli's eagles samples. The results showed a higher detection rate of real-time PCR compared to end-point PCR, with a melting temperature value of 83.45 degrees Celsius, and the identification of a trichomonad species reported for the first time in Bonelli's eagles.
Avian trichomonosis is a parasitic disease caused mainly by Trichomonas gallinae and other Trichomonas species. It can be asymptomatic, or it can produce a necrotic lesion in the upper digestive tract and spread to other organs, causing the death of the infected birds. In this study, we aimed to evaluate an adapted real-time PCR method for the diagnosis of different genotypes and species of avian oropharyngeal trichomonads. Fifty-six samples from the oropharynx of Bonelli's eagles (Aquila fasciata) obtained between 2018 and 2019 were analyzed using the real-time PCR and the end-point PCR, both targeting trichomonads ITS, and the results were compared by a coefficient of agreement. All positive samples were sequenced. The analysis showed a higher percentage of detection of real-time PCR ITS compared with end-point PCR ITS (64.3 vs 55.4%), and good agreement value (Kappa = 0.816). Melting temperature value for resulting amplicons of real-time PCR for avian trichomonads was 83.45 +/- 0.72 degrees C. Genotypes A, D, and III were found among the sequences. Moreover, Trichomonas gypaetinii, a common species in scavenger birds, is reported for the first time in Bonelli's eagles.
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