Journal
OPTICS LETTERS
Volume 47, Issue 22, Pages 6005-6008Publisher
Optica Publishing Group
DOI: 10.1364/OL.472691
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Funding
- Massner Lane Family Foundation
- Burroughs Wellcome Fund [CASI BWF 1014540]
- National Science Foundation [NSF CBET CAREER 1752011]
- Donaldson Charitable Trust Research Synergy Fund Award
- Georgia Institute of Technology
- National Institute of General Medical Sciences
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The study presents a simple technique for fast and label-free detection and grading of neutropenia using deep-UV microscopy of blood cells in microfluidic devices. The technique is cost-effective and can be used in low-resource, at-home, or point-of-care settings.
Neutropenia is a condition comprising an abnormally low number of neutrophils, a type of white blood cell, which puts patients at an increased risk of severe infections. Neutropenia is especially common among cancer patients and can disrupt their treatment or even be life-threatening in severe cases. Therefore, routine monitoring of neutrophil counts is crucial. However, the current standard of care to assess neutropenia, the complete blood count (CBC), is resource-intensive, time-consuming, and expensive, thereby limiting easy or timely access to critical hematological information such as neutrophil counts. Here, we present a simple technique for fast, label-free neutropenia detection and grading via deep-ultraviolet (deep-UV) microscopy of blood cells in polydimethylsiloxane (PDMS)-based passive microfluidic devices. The devices can potentially be manufactured in large quantities at a low cost, requiring only 1 mu L of whole blood for operation. We show that the absolute neutrophil counts (ANC) obtained from our proposed microfluidic deviceenabled deep-UV microscopy system are highly correlated with those from CBCs using commercial hematology analyzers in patients with moderate and severe neutropenia, as well as healthy donors. This work lays the foundation for the development of a compact, easy-to-use UV microscope system to track neutrophil counts that is suitable for low-resource, at-home, or point-of-care settings. (c) 2022 Optica Publishing Group
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