Journal
NUCLEIC ACIDS RESEARCH
Volume 50, Issue 16, Pages 9580-9595Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkac713
Keywords
-
Categories
Funding
- National Natural Science Foundation of China [32030064, 91940303, 31730110, 31570823, 31661143031, 31971367]
- National Key Research and Development Program of China [2018YFA0107602, 2021YFA1300503]
- Strategic Priority Research Program of Chinese Academy of Sciences [XDB38040100]
- StarryNight Science Fund at Shanghai Institute for Advanced Study of Zhejiang University [SN-ZJU-SIAS-009]
- Science and Technology Commission of Shanghai Municipality [17JC1404900, 18XD1404400]
- National Postdoctoral Program for Innovative Talents [BX20180336]
- Shanghai Super Postdoctoral Program
- CAS Pioneer Hundred Talents program (type A)
Ask authors/readers for more resources
This study reports a new RNA editing system that allows precise gene editing using a single engineered protein, without the need for auxiliary RNA. The system achieves high editing efficiency and low off-target effects, making it applicable for correcting disease-associated gene mutations.
Programmable RNA editing enables rewriting gene expression without changing genome sequences. Current tools for specific RNA editing dependent on the assembly of guide RNA into an RNA/protein complex, causing delivery barrier and low editing efficiency. We report a new gRNA-free system, RNA editing with individual RNA-binding enzyme (REWIRE), to perform precise base editing with a single engineered protein. This artificial enzyme contains a human-originated programmable PUF domain to specifically recognize RNAs and different deaminase domains to achieve efficient A-to-I or C-to-U editing, which achieved 60-80% editing rate in human cells, with a few non-specific editing sites in the targeted region and a low level off-target effect globally. The RNA-binding domain in REWIREs was further optimized to improve editing efficiency and minimize off-target effects. We applied the REWIREs to correct disease-associated mutations and achieve both types of base editing in mice. As a single-component system originated from human proteins, REWIRE presents a precise and efficient RNA editing platform with broad applicability.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available