Journal
CARBOHYDRATE RESEARCH
Volume 417, Issue -, Pages 81-88Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carres.2015.09.004
Keywords
Acinetobacter baumannii; Capsular polysaccharide structure; Polysaccharide gene cluster; Glycosyltransferase; K locus
Funding
- Russian Foundation for Basic Research [14-04-00657]
- National Health and Medical Research Council of Australia (NHMRC) [1026189]
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Neutral capsular polysaccharides (CPSs) were isolated from Acinetobacter baumannii NIPH190, NIPH201, and NIPH615. The CPSs were found to contain common monosaccharides only and to be branched with a side-chain 1 -> 3-linked beta-D-glucopyranose residue. Structures of the oligosaccharide repeat units (K units) of the CPSs were elucidated by 1D and 2D H-1 and C-13 NMR spectroscopy. Novel CPS biosynthesis gene clusters, designated KL30, KL45, and KL48, were found at the K locus in the genome sequences of NIPH190, NIPH201, and NIPH615, respectively. The genetic content of each gene cluster correlated with the structure of the CPS unit established, and therefore, the capsular types of the strains studied were designated as K30, K45, and K48, respectively. The initiating sugar of each K unit was predicted, and glycosyltransferases encoded by each gene cluster were assigned to the formation of the linkages between sugars in the corresponding K unit. (C) 2015 Elsevier Ltd. All rights reserved.
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