Establishment of Afgekia mahidolae BL Burtt & Chermsir in vitro culture and effect of elicitation on its bioactive compounds

Afgekia mahidolae is a rare plant species that possesses antioxidant, antimicrobial, and wound healing properties. This study aimed to establish the in vitro culture of A. mahidolae and investigate the effects of elicitors on their phenolic and flavonoid production, including the antioxidant activities. The established callus was prepared in the form of cell suspension cultures to determine the effect of elicitors. After elicitation for 3 days, A. mahidolae cell suspension cultures treated by 5 mu M salicylic acid or 100 mg/L yeast extract exhibited significantly higher levels of total phenolic and total flavonoid content than untreated cultures, which correlated to the antioxidant activities. In addition, the profiles of phenolic and flavonoid compounds in the callus and intact leaves of A. mahidolae were determined by LC-MS, which showed different phytochemicals. The findings of this study may encourage more sustainable development of A. mahidolae cultivation.

Automated summary

This study aims to establish the in vitro culture of A. mahidolae and investigate the effects of elicitors on their phenolic and flavonoid production. The findings demonstrate that cell suspension cultures of A. mahidolae treated with salicylic acid or yeast extract exhibit significantly higher levels of phenolic and flavonoid content and antioxidant activities.