LC-MS profile, in vitro acetylcholinesterase inhibitory, antibacterial and hemostatic properties of Ranunculus bullatus extract

The ethanol extract from aerial parts of Ranunculus were investigated for its chemical composition by LC-ESI-MS/MS technique, which allowed to identify a series of glycosylated flavonoids and one phenolic acid. RBEE extract showed acetyl-cholinesterase inhibition higher than the reference compound Galantamine at a concentrationof 200 mu g/mL. A moderate antibacterial activity of the extract was also obtained against Staphylococcus aureus ATCC43300, Citrobacter freundii ATCC8090, and Proteus vulgaris ATCC29905 at a concentration 100 mu g. Additionally, a good reduction in plasma coagulation time at 200 mu L was also observed for RBEE.

Automated summary

This study identified the chemical composition of the ethanol extract from aerial parts of Ranunculus, including a series of glycosylated flavonoids and one phenolic acid. The extract showed higher acetyl-cholinesterase inhibition compared to the reference compound Galantamine, moderate antibacterial activity against certain strains, and a reduction in plasma coagulation time.