Journal
NANOTECHNOLOGY
Volume 33, Issue 50, Pages -Publisher
IOP Publishing Ltd
DOI: 10.1088/1361-6528/ac9143
Keywords
graphene; polydimethylsiloxane (PDMS); scanning electron microscope (SEM); correlative light and electron microscopy (CLEM)
Funding
- Nano.Material Technology Development Program through the National Research Foundation of Korea (NRF) - Ministry of Science and ICT [NRF-2016M3A7B4027710, 2016M3A7B4900135]
- Focused Research Program of the Korea Research Institute of Chemical Technology (KRICT)
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In this study, we discovered that hydrophilic graphene can be used as an ideal imaging plate for biological specimens, providing excellent image quality and improved z-axis resolution. By functionalizing graphene with polydimethylsiloxane oligomer and processing it with oxygen plasma, hydrophilic graphene with minimal damage to graphene can be obtained.
In the present study, we showed that hydrophilic graphene can serve as an ideal imaging plate for biological specimens. Graphene being a single-atom-thick semi-metal with low secondary electron emission, array tomography analysis of serial sections of biological specimens on a graphene substrate showed excellent image quality with improved z-axis resolution, without including any conductive surface coatings. However, the hydrophobic nature of graphene makes the placement of biological specimens difficult; graphene functionalized with polydimethylsiloxane oligomer was fabricated using a simple soft lithography technique and then processed with oxygen plasma to provide hydrophilic graphene with minimal damage to graphene. High-quality scanning electron microscopy images of biological specimens free from charging effects or distortion were obtained, and the optical transparency of graphene enabled fluorescence imaging of the specimen; high-resolution correlated electron and light microscopy analysis of the specimen became possible with the hydrophilic graphene plate.
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