Journal
NANOTECHNOLOGY
Volume 33, Issue 48, Pages -Publisher
IOP Publishing Ltd
DOI: 10.1088/1361-6528/ac8bd8
Keywords
black phosphorus nanosheets; poly(allylamine hydrochloride); human epididymis protein 4; immunosensor; electrochemical biosensor
Funding
- National Natural Science Foundation of China [21874128]
- Jilin Provincial Science and Technology Development Project [20190201069JC]
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An electrochemical immunosensor based on BPNS/PAH nanocomposite modified electrode was developed for the detection of ovarian cancer biomarker HE4. The sensor showed good linear range, detection limit, selectivity, and specificity towards HE4.
In this work, an electrochemical immunosensor based on black phosphorus nanosheets (BPNS)/poly(allylamine hydrochloride) (PAH) nanocomposite modified glassy carbon electrode was developed for the detection of ovarian cancer biomarker HE4. PAH has been applied to retain BPNS in its original honeycomb structure and to anchor biomolecules electrostatically on the transducer surface. The as synthesized nanocomposite was characterized by zeta potential analysis, scanning electron microscopy, x-ray photoelectron spectroscopy, transmission electron microscopy, high-resolution transmission electron microscopy. Subsequently, the performance of the electrochemical immunosensor was evaluated through cyclic voltammetry, differential pulse voltammetry and electrochemical impedance spectroscopy. Under the optimal condition, the developed electrochemical immunosensor permitted to detect HE4 with a linear range of 0.1-300 ng ml(-1) and a detection limit of 0.01 ng ml(-1). The developed sensor exhibited good selectivity and specificity to HE4 with negligible interference effect from common biomolecules like bovine serum albumin, lysozyme, protamine, glucose, fructose, hemoglobin and fetal bovine serum. Further, practical application of developed electrochemical immunosensor was demonstrated in spiked human serum which showed satisfactory recovery percentages.
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