Investigation on the surface morphologies of reduced graphene oxide coating on the interfacial characteristics and electro-catalytic capacity of enzymatic glucose sensors

In this study, reduced graphene oxide (rGO) were subject to ultrasonic treatment to acquire varied morphologies, and the enzymatic glucose sensors were constructed by coating the rGO onto indium tin oxide electrodes and physically linking glucose oxidase to the rGO coatings. The effects of the surface morphologies of the rGO coatings on the interfacial characteristics and the electro-catalytic capacity of the enzymatic glucose sensors were systematically investigated. It turns out that, the rGO coating with a rough surface is more hydrophilic, and exhibits uniform glucose oxidase adsorption and higher electron migration rate at the solid/liquid interface between the analytical liquid and the working electrode. As a result, the corresponding glucose sensor shows excellent electro-catalytic capacity towards glucose with a broader linear range of 0-10.0 mM, a higher sensitivity of 38.9 mu A center dot mM(-1)center dot cm(-2), and a lower detection limit of 0.1 mu M (signal-to-noise ratio of 3). Additionally, the as-prepared glucose sensor exhibits excellent accuracy for detecting actual blood samples as well as superior resistance to interference from other substances (such as L-phenylalanine, urea, ascorbic acid, uric acid, NaCl, and KCl). These results establish the theoretical and experimental foundation for the application of rGO coating in the field of biosensors.

Automated summary

In this study, varied morphologies of reduced graphene oxide (rGO) were obtained through ultrasonic treatment, and enzymatic glucose sensors were constructed by coating rGO on indium tin oxide electrodes. The effects of different surface morphologies of rGO coatings on the performance of enzymatic glucose sensors were investigated. It was found that rGO coatings with rough surfaces exhibited better interfacial characteristics and higher electro-catalytic capacity, leading to glucose sensors with a broader linear range, higher sensitivity, and lower detection limit.