Journal
NANO RESEARCH
Volume 16, Issue 2, Pages 3215-3223Publisher
TSINGHUA UNIV PRESS
DOI: 10.1007/s12274-022-5035-6
Keywords
plasmonic enhanced fluorescence; electromagnetic coupling; SARS-CoV-2 antibodies; hepatitis B surface antigen; immunofluorescence microarrays
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This article introduces an improved method for enhancing fluorescence using plasmonic enhanced fluorescence (PEF) technology. By self-assembling gold nanoparticles (GNPs) and subsequent annealing-assisted regulation, the method achieves fluorescence enhancement and high density electromagnetic coupling in the near-infrared region. The method has high sensitivity and low detection limit, making it valuable for clinical detection and disease diagnosis.
Plasmonic enhanced fluorescence (PEF) technology is a powerful strategy to improve the sensitivity of immunofluorescence microarrays (IFMA), however, current approaches to constructing PEF platforms are either expensive/time-consuming or reliant on specialized instruments. Here, we develop a completely alternative approach relying on a two-step protocol that includes the self-assembly of gold nanoparticles (GNPs) at the water oil interface and subsequent annealing-assisted regulation of gold nanogap. Our optimized thermal-annealing GNPs (TA-GNP) platform generates adequate hot spots, and thus produces high density electromagnetic coupling, eventually enabling 240 -fold fluorescence enhancement of probed dyes in the near-infrared region, For clinical detection of human samples, TA-GNP provides super-high sensitivity and low detection limits for both hepatitis B surface antigen and SARS-CoV-2 binding antibody, coupled with a much-improved detection dynamic range up to six orders of magnitude. Wth fast detection, high sensitivity, and low detection limit, TA-GNP could not only substantially improve the outcomes of IFMA-based precision medicine but also find applications in fields of proteomic research and clinical pathology.
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