Journal
NANO LETTERS
Volume -, Issue -, Pages -Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.nanolett.2c02605
Keywords
cell membrane analysis; DNA probes; fluorescence imaging; lipid-DNA conjugates; lipid domains; membrane order
Categories
Funding
- NIH [R35GM133507]
- Alfred P. Sloan Research Fellowship
- Camille Dreyfus Teacher-Scholar Award
- UMass Amherst
- Paul Hathaway Terry Scholarship
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The cell membrane is a complex mixture of lipids, proteins, and other components that can selectively interact with each other to control cell signaling. This study introduces new types of DNA zipper probes, which can be used to measure cell membrane dynamic interactions and the formation of lipid domains.
The cell membrane is a complex mixture of lipids, proteins, and other components. By forming dynamic lipid domains, different membrane molecules can selectively interact with each other to control cell signaling. Herein, we report several new types of lipid-DNA conjugates, termed as DNA zippers, which can be used to measure cell membrane dynamic interactions and the formation of lipid domains. Dependent on the choice of lipid moieties, cholesterol- and sphingomyelin-conjugated DNA zippers specifically locate in and detect membrane lipid-ordered domains, while in contrast, a tocopherol-DNA zipper can be applied for the selective imaging of lipid-disordered phases. These versatile and programmable probes can be further engineered into membrane competition assays to simultaneously detect multiple types of membrane dynamic interactions. These DNA zipper probes can be broadly used to study the correlation between lipid domains and various cellular processes, such as the epithelial-mesenchymal transition.
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