Journal
MOLECULES
Volume 27, Issue 20, Pages -Publisher
MDPI
DOI: 10.3390/molecules27206779
Keywords
nanozyme; graphene quantum dot; hemin; peroxidase-like activity; colorimetric determination of glutathione
Funding
- National Natural Science Foundation of China [21904117]
- Zhejiang Provincial Natural Science Foundation of China [LY20B050007]
Ask authors/readers for more resources
Compared with natural enzymes, nanozymes have advantages such as good catalytic performance, high stability, and low cost. This study demonstrates the preparation of a nanozyme based on the dispersion of hemin by graphene quantum dot (GQD) and its application in colorimetric detection of glutathione (GSH). The nanozyme exhibits excellent peroxidase-like activity and can be used as a sensitive platform for the detection of GSH. The mechanisms underlying the nanozyme activity have also been investigated.
Compared with natural enzymes, nanozymes have the advantages of good catalytic performance, high stability, low cost, and can be used under extreme conditions. Preparation of highly active nanozymes through simple methods and their application in bioanalysis is highly desirable. In this work, a nanozyme based on dispersion of hemin by graphene quantum dot (GQD) is demonstrated, which enables colorimetric detection of glutathione (GSH). GQD was prepared by a one-step hydrothermal synthesis method. Hemin, the catalytic center of heme protein but with low solubility and easy aggregation that limits its catalytic activity, can be dispersed with GQD by simple sonication. The as-prepared Hemin/GQD nanocomplex had excellent peroxidase-like activity and can be applied as a nanozyme. In comparison with natural horseradish peroxidase (HRP), Hemin/GQD nanozyme exhibited a clearly reduced Michaelis-Menten constant (K-m) when tetramethylbenzidine (TMB) was used as the substrate. With H2O2 being the substrate, Hemin/GQD nanozyme exhibited a higher maximum reaction rate (V-max) than HRP. The mechanisms underlying the nanozyme activity were investigated through a free radical trapping experiment. A colorimetric platform capable of sensitive detection of GSH was developed as the proof-of-concept demonstration. The linear detection range was from 1 mu M to 50 mu M with a low limit of detection of 200 nM (S/N = 3). Determination of GSH in serum samples was also achieved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available