Journal
MOLECULES
Volume 27, Issue 17, Pages -Publisher
MDPI
DOI: 10.3390/molecules27175470
Keywords
Bupleurum chinense DC; drought stress; terpenoid backbone and triterpenoid biosynthesis; plant hormones; saikosaponins
Funding
- National Natural Science Foundation of China [82104329]
- special funds for starting scientific research of Henan University of Chinese Medicine [00104311-2021-1-41]
- National Key R&D Programme of China [2017YFC1702800]
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This study investigated the transcriptional changes of Bupleurum chinense under drought stress and found that it induced the accumulation of saikosaponins and increased the levels of abscisic acid, salicylic acid, and jasmonic acid. Additionally, differential genes related to terpenoid backbone and triterpenoid biosynthesis were up-regulated during drought stress. The study also identified possible key enzyme genes and transcription factors involved in the synthesis of saikosaponins. These findings have important implications for the cultivation and medicinal quality of Bupleurum chinense.
Bupleurum chinense is an important medicinal plant in China; however, little is known regarding how this plant transcribes and synthesizes saikosaponins under drought stress. Herein, we investigated how drought stress stimulates the transcriptional changes of B. chinense to synthesize saikosaponins. Short-term drought stress induced the accumulation of saikosaponins, especially from the first re-watering stage (RD_1 stage) to the second re-watering stage (RD_2 stage). Saikosaponin-a and saikosaponin-d increased by 84.60% and 75.13%, respectively, from the RD_1 stage to the RD_2 stage. Drought stress also stimulated a rapid increase in the levels of the hormones abscisic acid, salicylic acid, and jasmonic acid. We screened 49 Unigenes regarding the terpenoid backbone and triterpenoid biosynthesis, of which 33 differential genes were significantly up-regulated during drought stress. Moreover, one P450 and two UGTs are possibly involved in the synthesis of saikosaponins, while some transcription factors may be involved in regulating the expression of key enzyme genes. Our study provides a reference for the cultivation of B. chinense and a practical means to ensure the quality (safety and effectiveness) of B. chinense for medicinal use, as well as insights into the modernization of the China Agriculture Research System.
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