Optimized prime editing efficiently generates glyphosate-resistant rice plants carrying homozygous TAP-IVS mutation in EPSPS

Article Biotechnology & Applied Microbiology

Engineered pegRNAs improve prime editing efficiency

James W. Nelson et al.

Summary: Prime editing technology enhances editing efficiency by optimizing the structure of pegRNAs, preventing degradation of the 3' region from affecting system activity, without increasing the risk of off-target editing.

NATURE BIOTECHNOLOGY (2022)

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Article Plant Sciences

A design optimized prime editor with expanded scope and capability in plants

Wen Xu et al.

Summary: This study demonstrated that using N-terminal reverse transcriptase-Cas9 nickase fusion and introducing multiple-nucleotide substitutions in the reverse transcriptase template can significantly enhance the efficiency of prime editing in plants and human cells. The optimized approach achieved higher editing frequencies compared to the original editor, Prime Editor 3, showing the potential to make previously non-editable target sites editable and expand the capabilities of prime editing in the future.

NATURE PLANTS (2022)

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Article Biochemistry & Molecular Biology

Enhanced prime editing systems by manipulating cellular determinants of editing outcomes

Peter J. Chen et al.

Summary: The study found that DNA mismatch repair (MMR) hinders prime editing and promotes undesired byproducts, but the efficiency of substitution, small insertion, and small deletion edits can be enhanced by developing PE4 and PE5 prime editing systems. Strategic silent mutations near the intended edit and optimization of prime editor protein also contribute to improved editing outcomes.

CELL (2021)

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Article Biotechnology & Applied Microbiology

High-efficiency prime editing with optimized, paired pegRNAs in plants

Qiupeng Lin et al.

Summary: Designing prime binding sites with a certain melting temperature and using two pegRNAs can substantially enhance the efficiency of prime editing.

NATURE BIOTECHNOLOGY (2021)

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Article Plant Sciences

Identification of herbicide resistance OsACC1 mutations via in planta prime-editing-library screening in rice

Rongfang Xu et al.

Summary: A prime-editing-library-mediated saturation mutagenesis method was developed to expand herbicide-resistant mutations in plants efficiently. This method identified 16 types of herbicide-resistance-endowing mutations at conserved residues of OsACC1, demonstrating its advantage over base-editing-mediated mutagenesis.

NATURE PLANTS (2021)

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Letter Biochemistry & Molecular Biology