Journal
MOLECULAR NUTRITION & FOOD RESEARCH
Volume 66, Issue 23, Pages -Publisher
WILEY
DOI: 10.1002/mnfr.202200300
Keywords
butyrate; G-protein-coupled receptor 109a; macrophages; type 1 diabetes; type 1 cytotoxic T cell
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Funding
- Natural Science Foundation for Distinguished Young Scholars of Jiangsu Province [BK20200026]
- Collaborative innovation center of food safety and quality control of Jiangsu Province
- Fundamental Research Funds for the Central Universities [JUSRP221037, JUSRP22007]
- Jiangsu Province Six Summit Talents Program [YY-038]
- Wuxi Taihu Talents
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The role of GPR109a receptor in type 1 diabetes (T1D) and its underlying mechanisms were investigated in this study. It was found that GPR109a deficiency accelerated the development of T1D and activated the M1 macrophage polarization, disrupting the pancreatic immune homeostasis. Activation of GPR109a on macrophages by dietary components may provide a new strategy for preventing or treating T1D.
Scope Dietary fibers can alter gut microbiota and microbial metabolite profiles. SCFAs are produced by bacterial fermentation of fiber, mediating immune homeostasis through G-protein-coupled receptors (GPCRs). GPR109a, a receptor for niacin and butyrate, expressed by immune cells and non-immune cells, is a key factor regulating immune responses. However, the role and underlying mechanisms of GPR109a in type 1 diabetes (T1D) remain unclear. Methods and results Experimental T1D was induced by streptozotocin in GPR109a-deficient (Gpr109a(-/-)) and wild type mice. The study found that Gpr109a(-/-) mice were more susceptible to T1D with dysregulated immune responses, along with increased M1 macrophage polarization (from 10.55% to 21.48%). Further, an adoptive transfer experiment demonstrated that GPR109a-deficient macrophages promoted the homing of intestine-derived type 1 cytotoxic T cells to pancreas (from 18.91% to 24.24%), thus disturbing the pancreatic immune homeostasis in non-obese diabetic mice. Mechanistically, GPR109a deficiency promoted M1 macrophage polarization associated with the activation of suppressor of cytokine signaling 3-signal transducer and activator of transcription 1 signaling pathway. Conclusion The findings reveal that macrophage GPR109a deficiency accelerates the development of T1D. Activation of GPR109a on macrophage by dietary components may provide a new strategy for preventing or treating T1D.
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