Hydrocortisone-Containing Animal-Derived Food Intake Affects Lipid Nutrients Utilization

Scope As the tremendous increases in consumption of animal-derived food, endogenous hydrocortisone migrating along the food chain to organism arouses extensive attention. This study aims to investigate the cumulative impacts of dietary hydrocortisone intake and mechanistic understanding on metabolism of lipid nutrients. Methods and results A total of 120 porcine muscles samples with different concentrations of hydrocortisone are collected at three time points. An operational food chain simulation framework is constructed and 175 lipid molecules are identified by UHPLC-Q-Orbitrap HRMS. Compared to the control group, 66 lipid molecules are significantly different, including 17 triglycerides and 31 glycerophospholipids. Integrated analyses of lipidomics and proteomics indicate that hydrocortisone promotes adipose triglyceride lipase and hormone sensitive lipase activity to precondition for triglycerides hydrolysis. Quantitative lipidomics analysis shows the presence of hydrocortisone decreases the concentration of docosahexaenoic acid (3.66 +/- 0.15-3.09 +/- 0.12 mg kg(-1)) and eicosapentanoic acid (0.54 +/- 0.09-0.48 +/- 0.06 mg kg(-1)). A noteworthy increase of most saturated triglycerides concentration with the prolonging of time is observed. Conclusions Hydrocortisone originating from animal-derived food induces glycerophospholipids degradation and triglycerides hydrolysis through promoting adipose triglyceride lipase, hormone sensitive lipase, and phosphoglycerate kinase activity and further intervenes lipid nutrients utilization.

Automated summary

This study investigated the effects of dietary hydrocortisone intake on lipid metabolism. The results showed that hydrocortisone promoted triglyceride hydrolysis and decreased the concentration of certain fatty acids. Additionally, a significant increase in saturated triglycerides concentration over time was observed.