Journal
MOLECULAR NEUROBIOLOGY
Volume 59, Issue 11, Pages 6983-6992Publisher
SPRINGER
DOI: 10.1007/s12035-022-03014-y
Keywords
Alzheimer's disease; A beta(25-35); Ferroptosis; Oxidative stress; Senegenin
Categories
Funding
- Natural Science Foundation of Guangdong province [2014A030313394]
- Project of Science and Technology of Guangzhou [2014J4100098]
- Fundamental Research Funds for the Central Universities in China [21613401]
- Project of Science and Technology Plan in Guangzhou: City-University (Hospital) [202201020016]
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This study found that Sen exhibits strong neuroprotective activity against A beta(25-35) induced oxidative damage and lipid metabolic associated with ferroptosis.
Oxidative stress is one of the pathological mechanisms of Alzheimer's disease (AD), and ferroptosis has been determined to be involved in neurodegenerative diseases such as AD. Senegenin (Sen) prevents oxidative damage in nerve cells via a mechanism that may be highly related to ferroptosis. However, the mechanism of ferroptosis pathway involvement in AD is unclear. In this study, we established a model of PC12 cytotoxic injury induced by A beta(25-35), and we detected the level of oxidative damage, MMP, and ferroptosis-related protein expression. The results showed that, compared with control group, the level of ROS increased, GPX activities decreased, and MDA levels increased in A beta(25-35) group. A beta(25-35) could induce mitochondrial depolarization in PC12 cells and Fer-1 could not reverse this damage. WB revealed that A beta(25-35) group had increased ACSL4 and PEBP1 proteins, and decreased GPX4 protein. After adding Sen in the model, the level of oxidative damage was reduced, and mitochondrial depolarization was reversed compared with A beta(25-35) group. WB suggested that the expression of ACSL4 and PEBP1 proteins decreased, and the expression of GPX4 protein increased by Sen treatment. In conclusion, we found that Sen exhibits strong neuroprotective activity against A beta(25-35) induced oxidative damage and lipid metabolic associated with ferroptosis. Inhibiting nerve cell ferroptosis might facilitate the future development of strategies to AD.
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