4.5 Article

Cytotoxicity, apoptosis inducing activity and Western blot analysis of tanshinone derivatives from Stachys parviflora on prostate and breast cancer cells

Journal

MOLECULAR BIOLOGY REPORTS
Volume 49, Issue 9, Pages 8251-8258

Publisher

SPRINGER
DOI: 10.1007/s11033-022-07541-8

Keywords

Stachys parviflora; Tanshinones; Diterpenes; Cytotoxicity; Apoptosis; western blot

Funding

  1. Mashhad University of Medical Sciences [950763]

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The methanolic crude extract of Stachys parviflora and its sub-fractions were evaluated for their cytotoxic activities, with 1-hydroxy-tanshinone IIA showing the highest cytotoxicity. Further investigation revealed enhanced DNA fragmentation and altered protein levels in response to the methanolic extract and 1-hydroxy-tanshinone IIA.
Cytotoxic activities of methanolic crude extract of Stachys parviflora (Lamiaceae family) and its sub-fractions were primarily evaluated against human breast adenocarcinoma (MCF-7 and MDA-MB-231) and prostate (PC3) cell lines. The methanolic extract exhibited the highest activity, and was chosen for the isolation procedure. Four diterpenoid qui- nones, namely miltirone [1], tanshinone IIA [2], 1-hydroxy-tanshinone IIA [3], and cryptotanshinone [4] were isolated. Notably, this is the first report on the isolation and/or characterization of the mentioned diterpenoids from the Stachys genus. In this study, 1-hydroxy-tanshinone IIA [3] displayed the highest cytotoxicity among the isolated compounds. The mechanism of the cytotoxicity of methanolic extract and isolated compounds was further investigated by the utilization of propidium iodide staining (PI) assay. The results showed that the methanolic extract and 1-hydroxy-tanshinone IIA [3] enhanced DNA fragmentation in PC3 and MCF-7 cells. Moreover, the western blotting analysis demonstrated increasing and decreasing protein levels of Bax and Bcl2, respectively, and cleaved poly ADP-ribose polymerase (PARP). Further bioassay-guided phytochemical assessments of S. parviflora can be suggested as a promising approach for discovering potent bioactive secondary metabolites.

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