Journal
MICROCHIMICA ACTA
Volume 189, Issue 9, Pages -Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-022-05390-7
Keywords
African swine fever virus (ASFV); Carbon nanodot; Loop-mediated isothermal amplification (LAMP); Fluorescence
Categories
Funding
- National Natural Science Foundation of China [81772290]
- Chongqing science and technology commission [cstc2021jcyj-msxmX0608]
- Graduate Scientific Research and Innovation Foundation of Chongqing, China [CYB21070]
- Chongqing Technology Innovation and Application Development Special Project [CSTC2019jscx-gksbX0132]
- Research Project of General Administration of Customs [2019HK029]
- Research Project of Chongqing Customs [2020CQKY10]
- Chongqing Graduate Tutor Team Construction Project
- Analytical and Testing Center of Chongqing University for TEM
- Chongqing University
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In this study, a fluorescence biosensor based on carbon nanodots and loop-mediated isothermal amplification was established for highly sensitive detection of African swine fever virus (ASFV). The biosensor showed rapid, sensitive, low-cost, and simple detection of ASFV, with high accuracy in actual sample assays.
The spread of African swine fever virus (ASFV) caused huge economic costs, so early detection is particularly important. Here, we established a fluorescence biosensor based on carbon nanodots (CNDs) and loop-mediated isothermal amplification (LAMP) to ultra-sensitively detect ASFV. LAMP with high efficiency produced a large amount of pyro phosphoric acid and caused pH change in a short time. CNDs with strong light stability had a large fluorescence response at the emission wavelength of 585.5 nm to small pH change by the excitation wavelength of 550 nm. The biosensor realized turn-off-on mode for ASFV detection with the detection limit as low as 15.21 copies mu L-1. In addition, the biosensor had high accuracy in the actual sample assay. Therefore, the biosensor achieved rapid, sensitive, low-cost, and simple detection for ASFV. Moreover, the biosensor broadened the detection pathway of LAMP as a tool with great development prospect.
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