Journal
MICROCHIMICA ACTA
Volume 189, Issue 10, Pages -Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-022-05465-5
Keywords
Parkinson's disease; Gold nanoparticles; Surface plasmon resonance; Immunosensor; Catalytic deposition
Categories
Funding
- National Natural Science Foundation of China [62005156, 61875114]
Ask authors/readers for more resources
A nanozyme-based biosensing platform using self-assembled iron porphyrin mediated supramolecular modified gold nanoparticles was developed for the early diagnosis of Parkinson's disease. The sensor achieved sensitive detection of nitrated alpha-synuclein as a potential biomarker, showing promising results in distinguishing healthy individuals from PD patients using actual samples.
A self-assembled nanozyme of iron porphyrin mediated supramolecular modified gold nanoparticles (FpA) was fabricated to determine nitrated alpha-synuclein as the Tyr 39 residue (nT39 alpha-Syn) of a potential biomarker for early diagnosis of Parkinson's disease (PD). Mechanically, localized surface plasmon resonance (LSPR) and the mass effect caused by catalytic deposition of the nanozyme contributed to a cascade signal amplification strategy. The sensor allowed a signal amplification and selective nT39 alpha-Syn bioanalysis with a 1.34-fold enhancement by cascade amplified SPR signal and double specific recognition. The detection limit was 1.78 ng/mL in the detection range of 7-240 ng/mL. Benefiting from the excellent immunosensor, this method can distinguish healthy people and PD patients using actual samples. Overall, this strategy provides a nanozyme-based biosensing platform for the early diagnosis of PD and can be applied to detect other protein biomarkers, such as PD-L1.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available