Sensitive colorimetric detection of miRNA-155 via G-quadruplex DNAzyme decorated spherical nucleic acid

Rapid and sensitive detection of biomarkers enables monitoring patients' health status and can enhance the early diagnosis of deadly diseases. In this work, we have developed a new colorimetric platform based on spherical nucleic acid (SNA) and G-quadruplex DNAzymes for the identification of specific miRNAs. The simple hybridization between the target miRNA and two capture probes (capture probe 1 located at AuNP surface and free capture probe 2) is the working principle of this biosensor. The hybridization and duplex formation among probes and miRNAs led to a significant decrease in the intensity of color change. A linear relationship between the decrease of colorimetric signal and the amount of target molecules was witnessed from 1 to 100 nM for miRNA-155. Using this method, we were able to detect concentrations of miRNA-155 as low as 0.7 nM. Furthermore, the proposed sensing platform can be utilized profitably to detect miRNA-155 in real human serum samples. We further investigated the applicability of the proposed method in a microfluidic system which displayed promising results.

Automated summary

A new colorimetric platform based on spherical nucleic acid (SNA) and G-quadruplex DNAzymes has been developed for rapid and sensitive detection of specific miRNAs. The platform utilizes hybridization between target miRNA and capture probes to achieve detection. The method is capable of detecting miRNA-155 at concentrations as low as 0.7 nM and can be applied to real human serum samples.