Journal
MICROCHEMICAL JOURNAL
Volume 181, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.microc.2022.107829
Keywords
Electrogenerated chemiluminescence; Matrix metalloproteinase; Hemopexin-like domain; Inhibitor; Peptide; Sandwich
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Funding
- National Natural Science Foundation of China [21605120]
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A novel sandwich structure electrogenerated chemiluminescence biosensor was developed for determination of matrix metalloproteinase and its expressing cancer cells. The biosensor exhibited excellent sensitivity and a wide linear range.
A novel electrogenerated chemiluminescence (ECL) biosensor based on sandwich structure between target and inhibitory peptides was firstly developed for determination of matrix metalloproteinase and its expressing cancer cells. Here, target MMP-14 was used as model protein. Inhibitory peptide (AP1) was used as capture probe. Ruthenium complex (Ru) tagged inhibitory peptide (AP2) was used as signal probe (Ru-AP2) in this work. The biosensors were fabricated by covalently coupling Cys group of AP1 onto the surface of functional fullerenechitosan (C60-Chit) nanocomposite modified glassy carbon electrode. Upon binding of MMP-14, Ru-AP2 further specially bound to MMP-14 and a sandwich format formed, and increasing ECL signal was obtained. Under the optimal experimental conditions, the designed biosensor exhibited excellent sensitivity with a wide linear range of 0.05-7 pg mL(-1) and a detection limit of 0.008 pg mL(-1). The biosensor is demonstrated by detecting of living cells expressing MMPs (MDA-MB-231 cancer cells as model) and distinguish the expression MMPs in different cells. Our work firstly fabricated a sandwich ECL biosensor for determination of MMPs and MMPs relative cancer cells based on the interaction between peptide inhibitors and PEX of MMPs, which will provide a platform to study the mechanism of MMP and inhibitors.
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