Journal
MICROCHEMICAL JOURNAL
Volume 181, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.microc.2022.107763
Keywords
Chiral separation; beta-Cyclodextrin; Bovine serum album; Chiral stationary phase; Capillary electrochromatography; Open tubular column
Categories
Funding
- Science and Technology Project of Education Department of Jiangxi Province [GJJ201249]
- National Research Foundation of Korea (NRF) - Korean government (MSIT: Ministry of Science and ICT) [NRF2019R1A2C1010032]
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A capillary was immobilized with mixed beta-cyclodextrin and bovine serum albumin as the chiral stationary phase for enantioseparation of proton-pump inhibitors. The developed column showed excellent resolution and stability.
A capillary was co-immobilized with mixed beta-cyclodextrin and bovine serum album by chemical covalent coupling as the chiral stationary phase for the enantioseparation of proton-pump inhibitors in capillary electrochromatography. The chromatographic parameters including buffer concentration, pH value, organic modifier, and applied voltage were optimized. The separation performance of the developed column was evaluated by enantioseparating omeprazole, lansoprazole, pantoprazole, and esomeprazole with extremely high resolutions of 5.21, 7.11, 6.39, and 4.75, respectively. Moreover, the mixed chiral stationary phase exhibited a broader range and could separate more than 10 chiral analytes compared to the single-selector column. The relative standard deviations of the day-to-day and column-to-column repeatability were less than 4%, demonstrating excellent stability of the fabricated column. An open tubular capillary immobilized with the bovine serum album and beta-cyclodextrin as the chiral stationary phase is a promising prospect for separating chiral drugs in capillary electrochromatography because of the combination of the enantioselectivities of both individual selectors.
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