Journal
MICROBIAL CELL FACTORIES
Volume 21, Issue 1, Pages -Publisher
BMC
DOI: 10.1186/s12934-022-01898-y
Keywords
Arabitol; Glycerol; Wickeramomyces anomalus; Biorefinery; Central composite design
Categories
Funding
- University of Modena and Reggio Emilia [FAR2016DIP]
- Italian Ministry of University and Research (MUR)
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The yeast Wickerhamomyces anomalus WC 1501 has been shown to be an excellent producer of D-arabitol, with high conversion yield and productivity, using glycerol as a substrate.
Background: D-Arabitol, a five-carbon sugar alcohol, represents a main target of microbial biorefineries aiming to valorize cheap substrates. The yeast Wickerhamomyces anomalus WC 1501 is known to produce arabitol in a glycerol-based nitrogen-limited medium and preliminary fed-batch processes with this yeast were reported to yield 18.0 g/L arabitol. Results: Fed-batch fermentations with W. anomalus WC 1501 were optimized using central composite design (CCD). Dissolved oxygen had not a significant effect, while optimum values were found for glycerol concentration (114.5 g/L), pH (5.9), and temperature (32.5 degrees C), yielding 29 g/L d-arabitol in 160 h, a conversion yield of 0.25 g of arabitol per g of consumed glycerol, and a volumetric productivity of 0.18 g/L/h. CCD optimal conditions were the basis for further improvement, consisting in increasing the cellular density (3X), applying a constant feeding of glycerol, and increasing temperature during production. The best performing fed-batch fermentations achieved 265 g/L D-arabitol after 325 h, a conversion yield of 0.74 g/g, and a volumetric productivity of 0.82 g/L/h. Conclusion: W. anomalus WC 1501 confirmed as an excellent producer of D-arabitol, exhibiting a remarkable capability of transforming pure glycerol. The study reports among the highest values ever reported for microbial transformation of glycerol into D-arabitol, in terms of arabitol titer, conversion yield, and productivity.
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