Journal
METABOLIC ENGINEERING
Volume 73, Issue -, Pages 182-191Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymben.2022.08.002
Keywords
Salvia miltiorrhiza; Hairy root; SmWRKY34; SmbZIP3; Phenolic acids; Tanshinones
Categories
Funding
- National Natural Science Fund of China [82073963, 31571735, 81522049]
- Major Science and Technology Projects of Breeding New Varieties of Agriculture in Zhejiang Province [2021C02074]
- Zhejiang Provincial Ten Thousand Program for Leading Talents of Science and Technology Innovation [2018R52050]
- Zhejiang Provincial Program for the Cultivation of High-level Innovative Health Talents
- Zhejiang Chinese Medical University Research Foundation [2021JKZDZC06]
- Opening Project of Zhejiang Provincial Preponderant and Characteristic Subject of Key University (Traditional Chinese Pharmacology)
- Zhejiang Chinese Medical University [ZYAOXZ D2019004]
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This study identifies a new module, WRKY34-bZIP3, involved in ABA signaling that regulates the accumulation of phenolic acids and tanshinones in Salvia miltiorrhiza, providing new insights for metabolic engineering application in Salvia miltiorrhiza.
Phenolic acids and tanshinones are main bioactive compounds produced in Salvia miltiorrhiza widely used in treatment of cardiovascular diseases, which could be promoted by abscisic acid elicitation. However, the regulation mechanism remained to be elucidated. An ABA-inducible IIa WRKY transcription factor (TF) named SmWRKY34 exhibiting high homology with AtWRKY40 was isolated. SmWRKY34 exhibited a negative role on phenolic acids and tanshinones by directly regulating SmRAS and SmGGPPS. Moreover, ABA-responsive bZIP TF member named SmbZIP3 expressing significantly in SmWRKY34 transcriptome was screened. SmWRKY34 showed a negative regulatory role on SmbZIP3. SmbZIP3 acted as a positive regulator in the biosynthesis of phenolic acids and tanshinones by targeting SmTAT and two tanshinone-promoting TFs SmERF128 and SmMYB9b. Taken together, we identify a new module WRKY34-bZIP3 involved in ABA signaling that manipulates phenolic acid and tanshinone accumulation, shedding new insights in metabolic engineering application in S. miltiorrhiza.
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