Journal
MATRIX BIOLOGY
Volume 112, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.matbio.2022.08.007
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Funding
- Versus Arthritis [21447]
- University of Liverpool Crossley Barnes Bequest fund
- British Heart Foundation [FS/IBSRF/20/25032]
- Fondazione con il Sud grant within the Brains to South program [2018-PDR-0799]
- VELUX FONDEN [00014557]
- Danish Council for Independent Research Medical Science [DFF-4004-00471]
- LEO Foundation
- Novo Nordisk Foundation (BIO-MS) [NNF18OC0032724]
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This study investigates the role of low-density lipoprotein receptor-related protein 1 (LRP1) in the endocytosis process of articular cartilage. By analyzing the LRP1 interactome, the researchers identified numerous ligand candidates and confirmed their direct binding to LRP1. They also found that inhibition of LRP1-ligand interaction resulted in cell death and alterations in the secretome and transcriptional profile of human chondrocytes. The study highlights the specificity and extent of the chondrocyte LRP1 ligandome and identifies potential new therapeutic targets for osteoarthritis.
The low-density lipoprotein receptor-related protein 1 (LRP1) is a cell-surface receptor ubiquitously expressed in various tissues. It plays tissue-specific roles by mediating endocytosis of a diverse range of extracellular molecules. Dysregulation of LRP1 is involved in multiple conditions including osteoarthritis (OA) but little information is available about the specific profile of direct binding partners of LRP1 (ligandome) for each tissue, which would lead to a better understanding of its role in disease states. Here, we investigated adult articular cartilage where impaired LRP1-mediated endocytosis leads to tissue destruction. We used a top-down approach involving proteomic analysis of the LRP1 interactome in human chondrocytes, direct binding assays using purified LRP1 and ligand candidates, and validation in LRP1-deficient fibroblasts and human chondrocytes, as well as a novel Lrp1 conditional knockout (KO) mouse model. We found that inhibition of LRP1 and ligand interaction results in cell death, alteration of the entire secretome and transcriptional modulations in human chondrocytes. We identified a chondrocyte-specific LRP1 ligandome consisting of more than 50 novel ligand candidates. Surprisingly, 23 previously reported LRP1 ligands were not regulated by LRP1-mediated endocytosis in human chondrocytes. We confirmed direct LRP1 binding of HGFAC, HMGB1, HMGB2, CEMIP, SLIT2, ADAMTS1, TSG6, IGFBP7, SPARC and LIF, correlation between their affinity for LRP1 and the rate of endocytosis, and some of their intracellular localization. Moreover, a conditional LRP1 KO mouse model demonstrated a critical role of LRP1 in regulating the high-affinity ligands in cartilage in vivo. This systematic approach revealed the specificity and the extent of the chondrocyte LRP1 ligandome and identified potential novel therapeutic targets for OA. Crown Copyright (C) 2022 Published by Elsevier B.V.
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