Journal
CARBOHYDRATE POLYMERS
Volume 132, Issue -, Pages 146-155Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2015.06.059
Keywords
Bacterial cellulose; Alginate; Scaffold; Interconnected porous structure; Fibroblast; Freeze drying
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Funding
- Royal Golden Jubilee Ph.D program from Thailand Research Fund
- Ratchadaphiseksomphot Endowment Funds
- Chulalongkorn University of Postdoctoral Fellowship [RE5560530044-AM]
- Research Chair Grant, National Science and Technology Development Agency (NSTDA), Thailand
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A novel bacterial cellulose-alginate composite scaffold (N-BCA) was fabricated by freeze drying and subsequent crosslinking with Ca2+. The N-BCA then underwent a second freeze drying step to remove water without altering the physical structure. A stable structure of N-BCA with open and highly interconnected pores in the range of 90-160 mu m was constructed. The N-BCA was stable in both water and PBS. The swelling ability of N-BCA in water was approximately 50 times its weight, which was about 6.5 times that of the freeze dried bacterial cellulose pellicles. N-BCA demonstrated no cytotoxicity against L929 mouse fibroblast cells. For long-term culture, N-BCA supported attachment, spreading, and proliferation of human gingival fibroblast (GF) on the surface. However, under static conditions, the cell migration and growth inside the scaffold were limited. Because of its biocompatibility and open macroporous structure, N-BCA could potentially be used as a scaffold for tissue engineering. (C) 2015 Elsevier Ltd. All rights reserved.
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