Journal
MARINE DRUGS
Volume 20, Issue 10, Pages -Publisher
MDPI
DOI: 10.3390/md20100655
Keywords
cell factory; polyunsaturated fatty acids; omega-3 fatty acid; plant oils; fermentation; sulfur polymerization
Categories
Funding
- Australian Research Council [DP200100090]
- Flinders University Industry [(IPSG) 2020]
- Flinders University Industry partnership [DP200100090]
- Australian Research Council [DP200100090] Funding Source: Australian Research Council
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This study demonstrates a bioprocessing approach using low-molecular-weight compounds to manipulate the fatty acid profile in a thraustochytrid strain to produce desirable fatty acids. Interestingly, the addition of D-limonene repressed the production of polyunsaturated fatty acids. This work has industrial value and suggests the use of sulfur polymerization for the preparation of plant-like oils using tuneable thraustochytrid lipids.
In this study, we have demonstrated a bioprocessing approach encompassing the exogenous addition of low-molecular-weight compounds to tune the fatty acid (FA) profile in a novel thraustochytrid strain to produce desirable FAs. Maximum lipid recovery (38%, dry wt. biomass) was obtained at 1% Tween 80 and 0.25 mg/L of Vitamin B12. The transesterified lipid showed palmitic acid (C16, 35.7% TFA), stearic acid (C18, 2.1% TFA), and oleic acid (C18:1, 18.7% TFA) as the main components of total FAs, which are mainly present in plant oils. Strikingly, D-limonene addition in the fermentation medium repressed the production of polyunsaturated fatty acid (PUFAs). Sulfur-polymerization-guided lipid separation revealed the presence of saturated (SFAs, 53% TFA) and monounsaturated fatty acids (MUFAs, 46.6% TFA) in thraustochytrid oil that mimics plant-oil-like FA profiles. This work is industrially valuable and advocates the use of sulfur polymerization for preparation of plant-like oils through tuneable thraustochytrid lipids.
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