Campylobacter jejuni and other emerging Campylobacteraceae in retail beef liver - an underestimated potential source?

Bovine by-products, such as liver, could be an underestimated source of Campylobacter jejuni. Therefore, our aims were to evaluate the occurrence of C. jejuni and other Campylobacteraceae in retail beef liver and characterize their antibiotic resistance (ciprofloxacin, tetracycline, erythromycin and gentamicin) and potential genetic relationship by flagellin gene restriction fragment length polymorphism (flaA-RFLP) and multilocus sequence typing with clinical strains. Seventy-six out of 206 samples (36 center dot 9%) were positive for Campylobacter and related organisms. Arcobacter butzleri was the most frequently isolated species (21 center dot 8%), followed by C. jejuni (9 center dot 7%), C. fetus (7 center dot 8%) and C. coli (1%). The C. jejuni strains showed resistance to tetracycline (17 center dot 2%) or ciprofloxacin (6 center dot 9%), with only one strain resistant to both antibiotics. Meanwhile, 8 center dot 3% of ciprofloxacin resistance was observed in C. fetus. The other species showed no resistance. Most of the clonal complexes (CC) in which the C. jejuni genotypes were grouped (CC-21, 42, 48 and 52), coincided with genotypes of clinical strains previously reported in Chile. As such, this study provides evidence that beef liver could be an underestimated route for resistant C. jejuni to humans. Further studies should assess whether this food could play a role in the transmission of other emerging Campylobacteraceae such as those reported here.

Automated summary

This study evaluates the occurrence of Campylobacter jejuni and other related bacteria in retail beef liver and their antibiotic resistance. It suggests that beef liver could be an underestimated route for resistant C. jejuni transmission to humans.