Journal
CARBOHYDRATE POLYMERS
Volume 118, Issue -, Pages 30-35Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2014.11.012
Keywords
Bacille Calmette-Guerin; Sclerotia of Polyporus umbellatus FRIES; Polyporus polysaccharide; Bladder carcinoma
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Funding
- National Natural Science Foundation of Hebei [H2013201222]
- Hebei Administration of Traditional Chinese Medince [2013185, 2011106]
- Medical Science Foundation of Hebei University [2012A2001]
- National Natural Science Foundation of Shanxi [2014021037-7]
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There is growing interest in reducing Bacille Calmette-Guerin (BCG) side effects while keeping intact its therapeutic efficacy. In the present study, we evaluated the efficacy of Sclerotia of Polyporus umbellatus FRIES (Zhuling) and its main ingredient Polyporus Polysaccharide (PPS) to attenuate side effects of BCG therapy in vivo. The results show that bladder cancer development in model rats exhibited significantly reduced cancer invasiveness with Zhuling PPS combined with BCG. Flow cytometric (FCM) analysis showed expression of costimulatory molecules CD86, CD40, and TLR4/CD14 significantly increased with Zhuling PPS in combination with BCG. Similarly, immunohistochemical analysis revealed stronger CD86 and CD40 staining. Our findings show Zhuling PPS strongly reduced side effects and displayed synergistic effects during BCG instillation in rat bladder cancer models. The findings also suggest that the attenuation effect may result from direct activation of dendritic cell (DC) TLR4. (C) 2014 Elsevier Ltd. All rights reserved.
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