Journal
JOURNAL OF SEPARATION SCIENCE
Volume 45, Issue 22, Pages 4005-4011Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.202200608
Keywords
biogenic amines; deep eutectic solvents; high-performance liquid chromatography; mobile; phase additive
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Funding
- National Research Foundation of Korea (NRF) - Korean government (MSIT: Ministry of Science and ICT) [NRF-2019R1A2C1010032]
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This study proposes a novel method for the analysis of biogenic amines using deep eutectic solvents as additives in high-performance liquid chromatography. The method shows significant improvement in the separation of biogenic amines and exhibits excellent linearity, detection limit, precision, and accuracy.
Biogenic amines are present in large quantities in fermented foods and are a key marker for assessing food safety. This paper proposes a novel method for high-performance liquid chromatography analysis of biogenic amines using deep eutectic solvent as a mobile phase additive. After screening eight synthetic deep eutectic solvents and comparing them with several common additives, deep eutectic solvents based on choline chloride and ethylene glycol showed significant effects in improving the separation of biogenic amines. A Box-Behnken design of 17 runs was used to screen and optimize the key chromatographic parameters, resulting in an expected composition of the mobile phase of 0.73% deep eutectic solvent, 65% acetonitrile, and a column temperature of 28 degrees C. The proposed method exhibited excellent linearity (0.1-50 mu g/ml, R-2 >= 0.9987), limit of detection (0.007-0.031 mu g/ml), precision (1.28%-5.34%) and accuracy (87.2%110.6%). The method can be applied successfully to the separation and analysis of biogenic amines in cooking wine samples.
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